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NEW GENERATION LEDs
CoolLED's pE-300ultra offers intense, broad-spectrum LED
illumination for imaging most common fluorescent stains. Users have
access to both microsecond switching via multiple TTL inputs and the
ability to mount inline excitation filters. This, when paired with
today’s high performance multi band filter sets, facilitates imaging
traditionally done via a white light source and a filter wheel, with all
the benefits of LED.
The pE-300ultra is the most controllable member of the pE-300 Series. In addition to the many features of the pE-300lite and the pE-300white,
the system offers precise control over wavelength intensity and
shuttering. Until now these benefits have only been accessible to users
of high end, highly flexible illuminators such as the CoolLED pE-4000.
A broad spectrum of illumination is provided, covering the excitation bands of common fluorescent stains.
Triggering from multiple TTL inputs, coupled with the ability to
mount inline excitation filters, provides microsecond switching of
pre-filtered excitation light. This, when paired with today’s high
performance multi-band filter sets, facilitates imaging traditionally
done using a white light source and a filter wheel, with all the
benefits of LED, and most excitingly at speeds not previously so
The system comprises a pE-300ultra Light Source, Control
Pod, set of three Excitation Filter Holders and Power Supply. It can be
specified with a microscope adaptor for direct fit configuration or
with a 3mm diameter liquid light guide if there is a need to keep the
source of illumination remote from the microscope. An optional
pE-Universal Collimator and microscope adaptor can be selected for use
with a liquid light guide.
A benefit of the pE-300ultra is that stains can be viewed
either individually or in combination, without filter cube changes.
This makes it ideal for use with multi-band filter sets as the screening
process can be simplified when fewer filter cubes are used.
Independent control of the three LED channels means that the user can
control the level of excitation of each fluorescent stain independently
on a multi-stained sample, potentially removing the need for single band
filter sets altogether. For further information on improved multi-stain
testing, go to the Downloads tab.
An important additional feature of the pE-300ultra is that it includes CoolLED’s “Sequence Runner” multiple channel excitation mode. Users can define the order of their fluorophore capture using their pE-300ultra Control Pod, then the pE-300ultra
light source can accept a single TTL output from the experiment
set-up’s camera to initiate the step-through of a sequence of excitation
channels. This feature is independent of the individual channel TTL
inputs on the light source. This offers users the facility to run
through a sequence of excitation channels using a camera which has only a
With an expected lifetime in excess of 25,000 on hours and a comprehensive range of microscope adaptors, the pE-300ultra can
be fitted to most current and older microscopes and operate for many
years without aligning or replacing bulbs. The result is a safe,
convenient illumination system without any additional operating costs.
There are two pE-300ultra configuration options:
Direct-fit for connecting to a microscopes – by selecting
from a range of microscope adaptors which covers all current and most
older models. A simple once only adjustment will allow optimisation to
the optical path of the microscope.
Liquid Light Guide with a fixed 3mm diameter, liquid light
guide. An optional pE-Universal Collimator can be specified in
conjunction with a microscope adaptor if required.
FOR PRICE. STATING MICROSCOPE MODEL YOU HAVE